Previous study revealed that vdac2 gene was down-regualted in CsA-induced gingival overgrowth(GO) by differential display(DD)-PCR. Since VDAC2 is located at the outer membrane of mitochondria and implicated in apoptotis, the present study is to investigate whether CsA-induced GO is involving mitochondria-dependent apoptosis using RT-PCR and immunoblot in in vivo and in vitro.
In vivo, as a result, mRNA expression of vdac2 was decreased, followed by decrease the release of cytochrome c from mitochondria to cytosol. Subsequently, mRNA expression of caspase 9 and 3 were decreased, which meant CsA influenced cells to undergo against. In vitro system, CsA has dual effects on gingival fibroblast. Gingival fibroblast has a tendency to proliferate at low dose(500 ng/㎖), which is the same effects as in vivo, while to apoptosis at high dose(1500 ng/㎖).
Taken together, CsA affects gingival fibroblast through mitochondria-dependent apoptotic machinery. And GO by CsA is caused by the molecular events of undergoing against apoptosis.
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